Reddit mentions of Biomed Scientific Syringe Filters PVDF 13 mm Diameter 0.22 um Pore Size Non Sterile Pack of 10 pcs
Sentiment score: 0
Reddit mentions: 1
We found 1 Reddit mentions of Biomed Scientific Syringe Filters PVDF 13 mm Diameter 0.22 um Pore Size Non Sterile Pack of 10 pcs. Here are the top ones.
or
Syringe Filters PVDF:Hydrofilic PVDF membrane with Polypropylene(PP) housing,non sterileDiameter:13mm,pore size:0.22um,10/packConnectors:Inlet：Female luer,Outlet:male slip luerExcellent unit-to-unit and lot-to-lot reproducibility and consistencyMain features:Good heat--endurance and chemical stability ; PVDF can be either hydrophobic or hydrophilic; Good chemical compatibility;Applicable PH value 1-14
Specs:
| Number of items | 10 |
| Size | PVDF 13mm 0.22μm 10pcs |
| Weight | 0.0220462262 Pounds |
>How long can I keep the glc syringe before they go bad?
Few months, up to a year if kept in the fridge. I'd recommend putting it into a jar though since there will be air. Without air, yet being in a nutrient solution, the myc will die (not quickly, it'd take a long time) and any potential anaerobic bacteria will take over. The good thing about GLC is that there shouldn't be any anaerobic bacteria present in the first place.
>how exactly do i go about making an LC jar
I use a mason jar, drill two holes, one will have the self healing injection port (made with high temp silicone) and the other will be for the syringe filter that you secure with the same silicone. I use these filters. Whatever filter you use, just make sure it's synthetic and hydrophobic. The idea is that when you draw LC out, the volume has to be replaced with something (creates a vacuum) so the filter will filter the incoming air (HEPA is 0.3 micron, those are 0.22). I've tried micropore tape before, it works but when you shake the jar, it'll get on the tape and bacteria will grow.
I use 1tsp malt extract to 500ml water. (Note that there are some guides that recommend using 1tbsp malt to 300-500ml water. I prefer to have my mycelium in low nutrients until I'm putting them to grain). Heat the water on a stove to just below boiling, add the malt, stir it well, then pour into the jars. It's ok to have sediment, I actually like sediment since those are "anchors" for the mycelium to grow on. Fill it about 1/2 to a little over half, add a small piece of broken glass, pebble or a small screw that won't corrode from the LC (this will help break up the myc when swirling/shaking), wrap the top with foil and PC for 15-20m. Let it cool then add however much you want from the GLC. GLC is preferable as a starter since the idea is that a fully colonised jar won't have any contamination to carry over to the LC. Let it colonise for a week or two then you can put it in the fridge for longer storage. I made four master P. galindoi LC jars about two months ago and put them in the fridge. I checked them last week and they've barely grown.
If you can't get access to malt extract, you can also boil some potatoes and use that water as your LC. Many people have had success with using karo/dextrose/honey as their nutrient but I've never had much success with it. It's worth a shot, though, if you can't get malt. I wasn't able to find malt where I live so had to get it online.