#6 in Bottletop lab filters
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Reddit mentions of Corning 430049 Polystyrene Bottle Top Vacuum Sterile Filter, Nylon Membrane, 0.2 Micron, 45mm Bottle Neck Diameter, 500mL Capacity (Case of 12)
Sentiment score: 0
Reddit mentions: 2
We found 2 Reddit mentions of Corning 430049 Polystyrene Bottle Top Vacuum Sterile Filter, Nylon Membrane, 0.2 Micron, 45mm Bottle Neck Diameter, 500mL Capacity (Case of 12). Here are the top ones.
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- Bottle top vacuum filter is designed to fit plastic or glass media bottles with 45mm neck sizes
- Nylon (NY) membrane is naturally hydrophilic, surfactant-free, and offers the lowest extractables; membrane has a 0.22m pore size and a 33.2sq cm area
- Polystyrene filter funnel has a 500mL capacity and a 45mm diameter polyethylene neck adaptor with an angled hose connector that simplifies vacuum line attachment
- Individually packaged, sterile, and certified nonpyrogenic; designed for filtering and storing cell culture media, biological fluids, and other aqueous solutions
Features:
Specs:
| Number of items | 12 |
| Size | 45mm Diameter Bottle Neck, 33.2sq cm Nylon (NY) Membrane with 0.2µm Pore Size |
| Weight | 2.20462262 Pounds |
To illustrate what BST said...
Bottle Top filter example: http://www.amazon.com/Corning-430049-Polystyrene-Membrane-Diameter/dp/B005J5BVBI
Media Bottle: http://www.amazon.com/PYREX-500mL-Round-Storage-Bottles/dp/B004XR5TUM/ref=sr_1_1?s=industrial&ie=UTF8&qid=1421326439&sr=1-1&keywords=borosilicate+media+bottle.+45mm&pebp=1421326442468&peasin=B004XR5TUM
For autoclaving, in college we would just cover the top of the bottle with a small piece of aluminum foil and secure it with autoclave tape (which will also indicate when a specific temp has been reached)
Tape: http://en.wikipedia.org/wiki/Autoclave_tape
So I figured out why my bottle top filters wouldn't hold the vacuum, and it was simply because it wasn't airtight. I had to push the top against my thigh to get an airtight seal and only then did it hold a vacuum.
So basically, how do I make the top airtight? It only came with these crappy plastic dust covers. I've got these filters specifically, if it helps.
*Also, the filters are plastic, which I didn't factor in when I decided to buy them. Are they still good to use, considering they'll be under pressure? If so, how much pressure can I safely use?